Apr 20, 2009 the objective of this study was to assess the genetic variability within 43 wild lowbush blueberry clones, collected from 10 communities of four canadian provinces, and the cultivar fundy by using inter simple sequence repeat issr markers, with the hope to establish a reference set of lowbush blueberry germplasm for blueberry. Yu zhang1,2, haidong yan1, xiaomei jiang1, xiaoli wang3, linkai huang1. Issr anchored ssrpcr markers 12 are simple, rapid, inexpensive, and highly reproducible due to their primer length and to the high stringency achieved by the annealing temperature. Microsatellite markers were also employed to assess genetic diversity in 30 iraqi date palm cultivars 11. Issr markers for assessing dna polymorphism and genetic characterization of cattle. Inter simple sequence repeat issr polymorphism and its. Assessment of genetic diversity and structure of imperial.
The use of issr and rapd markers for genetic diversity among. A genetic analysis of species of pinanga palmae was conducted by using inter simple sequence repeat issr markers. The aflp markers used were only weakly correlated with issr r2 0. Genetic variation, population structure and linkage. Application of inter simple sequence repeat issr markers to plant genetics godwin, i. The number of alleles ranged from 2 to 8 per primer, with an average of 4. Pdf inter simple sequence repeat issr polymorphism and its. Ort 1 evaluation of genetic purity of f hybrid seeds in. Jan 01, 20 highlights association of issr markers with phytochemicals and antioxidant activity in valeriana jatamansi were established.
The grouping proposed by the issr markers was more coherent with the origin and the genealogy of the cultivars than that proposed by the rapd markers, and it can be considered the most. Abstract genetic variation among sweet, purple, and yellow passion fruit accessions was assessed using intersimple sequence repeat issr markers. Pdf rapd and issr markers for molecular characterization of. Relationships among pansies viola section melanium. Issr primers provided a total of 125 bands of which 94 74. Genetic structure of spanish populations of ceratitis. Among the tested primers, 17 produced reasonable amplification products with high intensity and pattern stability.
Pdf genetic variation among sweet, purple, and yellow passion fruit. Pdf nonanchored inter simple sequence repeat issr markers. Rapd random amplified polymorphic dna and issr inter simple sequence repeats markers were used to study dna polymorphism of thirty grewia optiva genotypes raised from seeds collected from various districts of himachal pradesh india and selected. The objective of this study was to evaluate the potential of this. They have a higher mutation rate than other areas of dna leading to high genetic diversity. Association of issr markers with some biochemical traits. Association of issr markers with some biochemical traits of.
Issr markers has also been used widely to study the genetic richness of durian in several countries in. Intersimple sequence repeat issr polymorphisms as a genetic marker system in cotton. Many of these studies have targeted speciwc citrus groups or sampled a few individuals of each taxon. Since issr primers inter simple sequence repeat primers also showed the suitability in genetic differentiation among different plant and animal genomes and also found useful in various silkworm races, these primers are selected for the present study.
For the issr markers technique, the dna from each cultivar was amplified with 16 issr primers in the following reaction. A total of 24 polymorphic primers 12 rapd and 12 issr were used. Issr marker with the wholegenome coverage, accuracy and reproducibility as well as robustness has proved to be a promising marker for genetic diversity analysis of many crops. A novel set of highly polymorphic chloroplast microsatellite and. A microsatellite is a tract of repetitive dna in which certain dna motifs ranging in length from one to six or more base pairs are repeated, typically 550 times. The number of clusters computed was the same for both molecular markers, with different sample composition. Molecular identification of echinococcus granulosususing issr. Genetic variability among 18 cultivars of cooking bananas and. Genetic diversity of the critically endangered thuja.
Inter simple sequence repeat issr pcr is a technique, which involvesthe use of microsatellite sequences as primers in a polymerase chainreaction to generate multilocus markers. Efficiency of inter simple sequence repeat issr markers. Issr markers for assessing dna polymorphism and genetic. Mean of polymorphism information content pic for each of the marker systems 0. Efficiency of rapd and issr markers system in accessing genetic. It can reveal variation in the numerous microsatellite regions by using primers that may be anchored with one or two nucleotides on either the 5 or 3 end of a repeat region and extend into the flanking region. Cluster analysis using upgma by rapd and issr markers obviously revealed distinct diversity between genotypes. An extended linkage map for watermelon based on srap. Keywords hydrangea macrophylla hyd1 in vitro regeneration. A total of 57 dna fragments loci separated by electrophoresis on agarose gels were detected and they ranged in size, from 150 to 900 bp. Pdf the application of issr markers in genetic variance. The aim of this study was to select issr intersimple sequence repeat primers capable of detecting genetic polymorphism among mimosa caesalpiniaefolia.
Analysis of the genetic diversity of eucalyptus cladocalyx. Genetic diversity analysis of walnut juglans regia l. Thus, nonanchored issr markers are a good choice for dna fingerprinting. The first issr studies were published in 1994 and focused on cultivated species see wolfe and liston, 1998. Efficiency of using rapd and issr markers in evaluation of. Issr markers as a tool for the assessment of genetic diversity in. Issr markers produced high rate of polymorphism depending upon polymorphic rate. Population authentication of the traditional medicinal plant. These characteristics make issr markers attractive, especially when the discussion is about species that have not yet been studied from the genetic point of view using molecular markers, such as m. Research article preliminary comparative analysis of.
Genetic diversity in spring wheat landraces from northwest of iran. But this is the first report on using issr markers to study the genetic diversity on p. Issr for intersimple sequence repeat is a general term for a genome region between microsatellite loci. Issr markers show differentiation among italian populations. Culley dominant markers, such as rapds and issrs, have become popular in molecular studies in recent years. Optimization of issr markers for molecular dna fingerprinting in aquilaria sp. So, this study aimed to distinguish and determine the differences and similarity among o. Nagaraju2, 1division of plant biotechnology, institute of forest genetics and tree breeding, coimbatore 641002, india. Population genetic analysis of issr data theresa m. Poerba ys, ahmad f 2010 genetic variability among 18 cultivars of cooking bananas and plantains by rapd and issr markers. Issr molecular markers for the study of the genetic. Analysis of genetic diversity in foxtail millet setaria.
Issr are single primer designed from ssr and amplified region between two ssrs. Total phenolics, flavonoids contents and antioxidant activity measured by three different in vitro. In addition we have performed dna ngerprinting of the prospective sample of the variety q. Out of 44 issr markers, ten markers produced clear, reproducible bands with a mean of 5. Cluster analysis classified the populations into 5 groups for both dna markers. Issr inter simple sequence repeats as molecular markers to. Microsatellites occur at thousands of locations within an organisms genome. Pdf association of issr markers with some biochemical. Genetic diversity in barley from west china based on rapd. Issr markers are more reliable than rapd because of the longer primers and the higher annealing temperature of issr than rapd. Two separate genetic linkage maps for chinese silver birch based on intersimple sequence repeat issr and amplified fragmentlength polymorphism aflp were constructed by a pseudotestcross mapping strategy. Assessing genetic diversity and population structure in a. Mar 18, 2005 the availability of a variety of dna markers, such as restriction fragment length polymorphism rflp, amplified fragment length polymorphism aflp, random amplified polymorphic dna rapd, simple sequence repeat ssr and intersimple sequence repeat issr has enabled researchers to investigate genetic diversity among various plant species across natural populations 15. Intersimple sequence repeat issr polymorphisms as a genetic.
Reports using rapd markers are still limited, and no report has been published with issr, ssr, or srap markers to test cabbage seed purity crockett et al. Issr and ssr markers for determining genetic relationships. As an alternative to isozymes, these types of markers are especially attractive given their hypervariable nature, the vast numbers of loci that can be examined, and the small. Issr markers are easy to use, cheap, and less demanding compared to other dominant markers. The use of issr and rapd markers for detecting dna. Pdf rapd and issr markers for molecular characterization. Since 1994, a new molecular marker technique called inter simple sequence repeat issr has been available zietkiewicz et al. Issr inter simple sequence repeats as molecular markers to study genetic diversity in tarantulas. Detection of genetic variations in orobanche crenata using. Issrs are now being applied to natural populations to address issues such as hybridization. Therefore, issr has been widely used for population genetic analysis of various plant species, including many rare or endemic plants, neopicrorhiza scrophulariiflora 17, swertia tetraptera 18, michelia coriacea 19, etc. Microsatellites are often referred to as short tandem repeats.
Application of aflp, rapd and issr markers to genetic mapping of european and japanese larch. Genetic similarity among strawberry cultivars assessed by. Download fulltext pdf download fulltext pdf download fulltext pdf download full. The rapd primers generated 204 amplification products and the issr primers produced 178 fragments, 190 and 173 of which were polymorphic, respectively.
The best markers for genome mapping, marker assisted selection, phylogenic studies, and crop conservation have low cost and labour requirements and high reliability. Development of issr markers for genetic diversity studies. The white backed planthopper, sogatella furcifera hemiptera. Thus the aim of this study was to select issr primers capable of detecting genetic polymorphisms in m.
Genetic analyses of casuarinas using issr and fissr markers. There are few reports on the use of issr markers in passiflora, however, some authors reported high polymorphism level in their work with species of this genus using such markers. Inter simple sequence repeat issr markers were used to study the genetic diversity of 18 spring growth type landraces from. The complementary sequences to two neighboring microsatellites are used as pcr primers. Assessment of genetic diversity among iranian aegilops. Genetic analyses of casuarinas using issr and fissr markers r. The study of genetic diversity of daemonorops draco p.
The issr markers showed that the lowest genetic polymorphism was 0. Research article biogeographical variation and population. Issr primers used in a genetic diversity study of five passiflora species in campos dos goytacazes. Primers used for issr markers are single and relatively long, so that these markers can detect variations at as many genetic loci as possible, which is useful in demonstrating a high level of polymorphism bornet and branchard, 2001. Estimation of the degree of diversity for some iraqi wheat. Sequence repeat issr molecular markers for genetic diversity studies, as well as to test the efficiency of this approach in. Agrotechnology and biosciences division, malaysian nuclear agency, bangi, selangor. Issr markers have numerous advantages that make them easy to be applied in several crop studies including high reproducibility. Inter simple sequence repeat issr markers for genetic. There was no direct relationship between the marker groups when the two types of markers were compared. Therefore, issr molecular markers ubc 827, 840, 844, 857, 859, 860 and 873 are considered.
Two floral characters, the upturned side petals and the large size of pollen grains appear to be synapomorphies in melanium. Optimization of issr markers for molecular dna fingerprinting. A high level of polymorphism was found with both rapd and issr markers, and the mean polymorphism information content pic values were 0. Polymorphic issr markers ranged from 0 ubc841 to 12 ubc890providing amean of6. Issr markers are highlypolymorphic and are useful in studies on genetic diversity, phylogeny, genetagging, genome mapping and evolutionary biology. An extended linkage map for watermelon based on srap, aflp. This study was done to assess the molecular diversity of 36 accessions 18 cultivars of the plantain and. Genetic structure of colletotrichum gloeosporioides. The results showed low levels of genetic diversity within populations he 0.
Genomewide novel genic microsatellite marker resource. Genetic diversity of the planthopper, sogatella furcifera in. The in vitro regeneration protocol is potentially useful for the genetic transformation of hydrangea macrophylla hyd1. Efficiency of rapd and issr markers for the detection of. Sequences of the nuclear region its and the variable molecular markers issr were used to estimate the phylogeny of the section melanium of the genus viola. A comparative analysis of rapd and issr markers for assessing. Ssr markers encoded by functionally relevant parts of a genome are. Infecting papaya inferred by multilocus issr markers.
In this study, intersimple sequence repeats issr markers were applied to assess genetic diversity and genetic relationships of 14 cultivars of durian durio. The application of issr markers in genetic variance. Nonanchored inter simple sequence repeat issr markers. It is a simple and quick methodthat combines most of the advantages of microsatellites ssrs andamplified fragment length polymorphism aflp to the universality ofrandom amplified polymorphic dna rapd. Development of issr markers for genetic diversity studies in. Genetic analysis of selected sargassum fusiforme harvey. Issr markers generate a large number of polymorphic fragments per primer fang and roose 1997. Issr molecular markers for the study of the genetic diversity. Because of the multilocus fingerprinting profiles obtained, issr analysis can be applied in studies involving genetic identity, parentage, clone and strain identification, and taxonomic studies of closely related species. Total bands were obtained in fingerprints by 11 issr markers. These studies demonstrated the hypervariable nature of issr markers. Genetic diversity of torch ginger etlingera elatior.
Use of issr markers for strain identification in the button. Sixteen iraqi varieties were screened with issr 10, srap, and rapd markers kits k, l, m and n operon technology, alameda, ca. Genetic diversity analysis among collected purslane. Among 97 issr markers, only three issr markers exhibited significant p aug 24, 2020 issr markers are also quick and easy to handle and more informative for the evaluation of genetic diversity korbin et al. Use of issr markers for cultivar identification in durum wheat.
Jan 01, 2015 the userfriendly nature of issr markers was successfully exploited in many crop species for a better understanding of the genetic diversity, domestication process and geographic divergence and distribution. Comparative analysis of genetic diversity in indian bitter. Pdf genetic linkage maps of betula platyphylla suk based. Towards establishing association of issr markers with antioxidant activity in valeriana jatamansi, 5 distantly located populations were analysed.
The issr method has been used in several palm species. The study indicates that the issr markers produce both reliable and reproducible bands because the annealing temperature and sequence of issr primers are extended qian et al. Several dna markers have been used to detect the genetic variation among date palm cultivars, selecting of molecular markers for their specific purposes is the main challenge for researchers, the aim of this study was to evaluate the efficiency of rapd and issr markers in assessing genetic diversity of date palm. Pdf genetic linkage maps of betula platyphylla suk based on. We confirm that the socalled pansies form a derived and monophyletic group. Diversity index value permits to evaluate the discri minating ability of each marker and is determined as follows.
Issr markers for assessing dna polymorphism and genetic characterization of cattle, goat and sheep populations nahid askari1, mohammadreza mohammad abadi2, amin baghizadeh3 1department of animal science, ramin agricultural and natural resources university, p. In addition, there was a significant genetic differentiation among provenances. Genetic diversity of mango cultivars estimated using scot. Issr markers as a tool for the assessment of genetic passi. The average polymorphism level with the issr markers 97. Issr markers as a tool to distinguish idt and sss populations. The average marker index mi values derived from the three different marker systems differed dramatically, indicating that they vary in their discriminatory power aflp issr rapd. Among these markers, rapd primers generated 987 amplification products of which 719 were polymorphic and issr. Since 1994, a new molecular marker technique called inter simple sequence repeat issr. In the present study, intersimple sequence repeat issr markers were employed to investigate the genetic diversity and differentiation of 47 populations sampled from 14 prefectures of the greater mekong subregion.
Ssr markers have been developed for the yellow passion fruit oliveira et al. The objective of this work was to evaluate the potential of issr markers for genotype identification in common button mushroom, a. Issr markers as a tool for the assessment of genetic. The dendrograms constructed using rapd and issr marker. Pdf issr markers as a tool for the assessment of genetic. The linkage map contains 360 dna markers distributed on 19 linkage groups.
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